When general sperm parameters such as – motility, concentration, morphology have been within the normal range, sperm may still be the cause of unsuccessful results after artificial insemination procedure. Sperm DNA fragmentation is expressed as a rupture of DNA strand in both or just one DNA helix. If the rupture affects genes which are vital for embryo and implantation development then during some stage the embryo may not survive.
Possible causes of DNA fragmentation:
- Oxidative stress
- Drug use
- Environmental pollution (heavy metal, air pollution)
- Elevated testicular temperature etc.
DNA fragmentation test is recommended for patients with:
- Unexplained Infertility
- Stoppage of embryo development
- Poor blastocyst development
- Multiple unsuccessful treatment cycles
- Pregnancy interruption in short period of time (miscarriages)
- Varicocele etc.
In some cases Sperm DNA fragmentation may be reduced by changing way of life and habits, also using antioxidants in the nutrition which will liquidate the free radicals as well as will treat the diseases causing the DNA fragmentation.
Sperm DNA fragmentation test is a laboratory diagnostic tool. Which based on the processing of sperm sample with various reagents and visualisation in the fluorescence microscope.