New ground-breaking IVF Technologies
Medicine is always moving forward. Every year, huge sums of money are spent on scientific research and development for the benefit of mankind. The sphere of reproduction is no exception. Thanks to new technologies, experts have learned the way to select reproductive cells, by choosing only best quality cells, thereby increasing the chance of pregnancy.
You can delay childbirth, if the circumstances require. Modern methods of cryoconservation (vitrification) allow a delay of parenthood because of social reasons as well as medical reasons – cancer or other disease treatment.
Future parents now can be sure about the health of their baby, especially if one of the family members has a genetic disease. Modern technology can to check all chromosomes and detect deviations at the stage of the embryo.
Created different mediums, synthesized more effective medicines and new methods of treatment, increasing the likelihood of better result.
All of these are modern advances in artificial insemination – this is another step for anyone who want to experience joy of parenthood. Thanks to advances in the field of reproduction, you have the chance to find happiness by becoming a mother or father.
The Emergence of The Blastocyst (Hatching)
During the first days of development, the human egg and embryo are surrounded by a protein shell (zona pellucida) - a kind of analogue of the bird's egg shell.
To guarantee the successful implantation (attachment) to the uterus, the embryo must 'hatch" from its protein shell – this is called hatching. In case of Artificial Insemination, an embryologist makes an opening in the shell of the Day 5 embryo - and this is called Assisted Hatching.
Sometimes it is considered that problems with hatching may cause problems with the occurrence of pregnancy in some couples. But Assisted Hatching can help to improve pregnancy chances for some groups of patients.
Assisted embryo hatching is a micromanipulation during which embryologists, before transferring the embryo into the uterus, open the embryo's shell (zona pellucida), helping it to "hatch".
This will make the process of natural hatching and implantation easier. Assisted hatching is required for the embryos before the testing for genetic abnormalities (PGT), as well as for Frozen Embryo Transfer (FET).
What are the Advantages of Blastocyst Culture?
Nowadays, it is possible to grow embryos in the laboratory until they reach the blastocyst stage – the embryo is cultured for five days, at which point it reached its highest stage of physiological development, after which it may be transferred into the uterus.
Some scientists consider that after this manipulation there is a higher chance for the occurrence of pregnancy.
By culturing embryos for a longer period of time, it makes it possible to choose the best ones for transfer into the uterine cavity.
Array Comparative Genomic Hybridization (aCGH) CGH Microarray
Preimplantation Genetic Screening (PGS) by aCGH
Within the IVF cycle, iVF Riga clinic offers unique examinations of embryos before their transfer to the uterus: preimplantation genetic screening by microarray-based comparative genomic hybridization (aCGH). This method provides high accuracy for the identification of chromosomal abnormalities prior to embryo transfer and implantation. In the near future, genetic analysis will allow for obtaining positive IVF results in the first cycle, eliminating failed attempts and termination of pregnancy after embryo implantation.
Cult-Active – an innovative method used in order to activate the Eggs (Oocytes) after fertilization
Recommended in cases with low amount of fertilized eggs in previous Artificial Insemination cycles
Cult-Active is a new method which is used in patients who have a low amount of fertilized egg cells (oocytes) during previous Artificial Insemination cycles after ICSI (intra-cytoplasmic sperm injection). It indicates that eggs are immature. Oocyte (egg) activation is initiated by protein contained in sperm. During egg activation, there is a fast increase in intracellular concentration of calcium.
After ICSI (intra-cytoplasmic sperm injection), fertilized eggs are placed in the Cult-Active medium for 15 minutes, which helps to activate an egg. Thereafter, an increasing amount of fertilized eggs emulate the biochemical process of egg and sperm fertilization. Calcium ionophore contained in Cult-Active medium- connects calcium ions and delivers them to an egg, thus having activated them.
EmbryoGen – A New Option in IVF
Artificial insemination is a multi-stage process during which every step is the most important one. Until present it was possible to control and ensure optimal conditions from the moment of egg collection until embryo transfer. Today with the help of the new EmbryoGen medium it is possible to improve the development of an embryo also in the uterus of a female. Embryos are cultivated in this medium until the third day, and according to the latest researches, it significantly improves the level of cell communication between the mother and embryo. Women after the age of 35 have a higher risk of not carrying a child successfully, it is recommended for those patients to apply EmbryoGen.
EmbryoGen's composition has signal molecules which are also found in the mother's tissue. These signal molecules improve the level of cell communication between the mother and embryo, thus protecting the embryo from stress and making it stronger at the beginning of implantation and at its end. One of the reasons for a miscarriage is an immune response from a woman's body to the embryo as a foreign object. EmbryoGen is improving this communication between the mother's and child's cells, which further improves the embryo implantation process.
- Repeated implantation failure (2 or more)
- Repeated preclinical (biochemical) pregnancy loss (2 or more)
- Recurrent miscarriage (2 or more)
- Other unexplained infertility
Embryo Glue – A New Hope For Couples With Failed IVF
Embryo Transfer (placement of an embryo into the uterus of a female) is the last and one of the most important steps in Artificial Insemination. From the Egg Collection procedure until the last stage (Embryo Transfer) – Embryo Laboratory is ensures optimal conditions and environment for development of the embryo. After Embryo Transfer it depends only on the embryo and its ability to attach to the uterus of the female. Currently, with the new EmbryoGlue-technique it is possible to help the embryo implant in the uterus.
EmbryoGlue – is a specially designed medium for usage in embryo transfer which improves implantation ability of the embryo. This medium is rich with hyaluronic acid, carbohydrates and amino acids, which when applied together work as a binding agent at the moment of embryo implantation in the uterus. Hyaluronic acid is a substance found naturally in the human body. Scientific research show that at the moment of embryo implantation the level of hyaluronic acid is very high, and this can help the embryo to become implanted better.
Raised level of hyaluronic acid in the EmbryoGlue medium (in which the embryo is placed before the transfer) improves the ability of the embryo to get attached in the uterus of the female.
PICSI – Detailed Sperm Selection Technique
ICSI (Intra-Cytoplasmic Sperm Injection) is defined as a single sperm injection into the cytoplasm of an egg. The technique requires a single sperm with the best morphology and motility to be injected into the cytoplasm of an egg. Until now, visual evaluation of a single sperm has been the only available method for choosing the best sperm cell for egg insemination. Embryologists often have to deal with immature sperm cells. Such effects from these cells are evaluated at the same time during embryo development. Sperm cells with good morphology and motility may have other defects and may not be suitable for visual evaluation, etc. – DNA fragmentation, late development. Those defects affect the quality and development of the embryo as well as the possibility of miscarriage.
Using the latest PICSI technique (injection of the physiologically best sperm cell into the cytoplasm of an egg) it is possible to select sperm cells without visible defects. This method improves the selection of sperm cells by up to 98%. In this technique, sperm cells are placed into hyaluronic acid which will attach to mature sperm cells. Only completely mature sperm cells which have reached the last stage of spermatogenesis have developed attachment receptors for hyaluronic acid. Immature sperm cells have high DNA fragmentation, aneuploidy (abnormal number of chromosomes) and immature cytoplasms. Using the latest PICSI technique will increase the possibility of getting a successful pregnancy.